Part:BBa_K175027:Experience

Characterization of BBa_K175027

Gel electrophoresis of BBa_K175027 in pSB1AK3 backbone. Lane 1: undigested (supercoiled) plasmid. Lane 2: BglI digested part, expected size = 3219bp. Lane 3: BglI and I-SceI restricted part, expected band lenghts are 2275 and 944bp. Expected lenghts correspond to the bands observed in the gel.

This biobrick was successfully sequenced and tested for functionality. Sequencing results can be found on the part page. Functionality was assessed using commercially available I-SceI restriciton enzyme. The plasmid was purified and subjected to a digestion with BglI alone and BglI plus I-SceI. The latter would theoretically result in two fragments of 2275 and 944bp.

The results in the adjecent image show that the digestion indeed results in the expected band pattern. This shows that the restriction site works as expected.

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